Sample Removal Procedure ------------------------ Due to several changes following the APS-Upgrade, users must adhere to the following procedure to safely remove their samples and reset the PE press for the next sample. .. warning:: Failure to follow these instructions can lead to damage of beamline equipment and loss of experimental time. .. important:: Please ensure the collimator tip is in the "out" position before beginning this procedure to avoid bumping the tip. If the tip is bumped, realignment can take several hours by the beamline staff. Sample Removal ^^^^^^^^^^^^^^ After following the decompression steps outlined in :ref:`Pressure control ` as well as the steps to disable and interlock the heater power supply as explained in :ref:`Heating control `, follow the following procedure. 1. Move the collimator tip to the "out" position 2. Put on safety glasses 3. Open the PE press line valve 4. Remove the bottom anvil with magnets 5. Put the sacrificial aluminum block inside the PE press .. figure:: /images/sample_removal/aluminum_block.png :alt: aluminum_block :width: 720px :align: center | 6. Remove the hydraulic reset piston from the red 3D printed holder 7. Mount the reset piston in the PE press by aligning the holes in the reset jig with the 6 shoulder bolts on the top of the PE press. Once the holes are aligned, drop the jig into the press and rotate the jig clockwise to lock it in place. .. figure:: /images/sample_removal/reset_jig_installation.png :alt: reset_jig_installation :width: 720px :align: center | 8. Lower the spring-loaded alignment pin to prevent the jig from turning during the reset process .. figure:: /images/sample_removal/alignment_pin.png :alt: alignment_pin :width: 720px :align: center | 9. Locate the hydraulic line and connect it to the reset piston. Pull down on the quick connect collar and insert the hydraulic line, then release the quick connect collar and ensure the hydraulic line is securely attached. .. figure:: /images/sample_removal/hydraulic_line_connection.png :alt: hydraulic_line_connection :width: 320px :align: center | 10. Locate the yellow hand hydraulic pump mounted under the second search box on the upstream wall of the hutch. .. figure:: /images/sample_removal/pump_cropped.jpg :alt: pump_cropped :width: 320px :align: center | 11. Ensure the pressure release knob is fully closed (turn it to the right). .. figure:: /images/sample_removal/closed_release_valve.png :alt: closed_relief_valve :width: 320px :align: center | 12. Pump oil until the pressure gauge shows 500 psi of pressure. DO NOT EXCEED 500 PSI OF PRESSURE. .. figure:: /images/sample_removal/pressure_gauge.jpg :alt: pressure_gauge :width: 320px :align: center | 13. The piston will engage with the sacrificial aluminum block inside the PE press and begin to push the piston of the PE press down. After a few seconds, the pressure on the gauge of the reset pump will begin to drop. Pump a small amount of oil until the pressure reaches 500 psi again. .. figure:: /images/sample_removal/piston_engaging.png :alt: piston_engaging :width: 720px :align: center | 14. Over the next few minutes, alternate between maintaining the pressure at 500 psi and visually monitoring the PE press piston. After 3-4 minutes, the piston of the PE press should be fully retracted. .. figure:: /images/sample_removal/PE_piston_retraction.png :alt: PE_piston_retraction :width: 720px :align: center | 15. Once the piston is retracted, open the pressure release valve by turning the knob to the left. The pressure on the gauge will drop to 0 psi and the reset piston will be retracted. .. figure:: /images/sample_removal/open_release_valve.png :alt: open_release_valve :width: 320px :align: center | 16. Push the handle of the pump up towards the body of the pump and close the pressure release knob by turning it to the right. .. figure:: /images/sample_removal/closed_release_valve.png :alt: closed_release_valve :width: 320px :align: center | 17. Disconnect the hydraulic line from the reset piston by pulling down on the collar of the quick disconnect fitting and removing the line. Use a paper towel to catch the small amount of oil that will drop from the line after it is disconnected. Carefully wipe the line clean and store it out of the way behind the PE press. 18. Close the line valve of the PE press on the User Control Screen. If you fail to do this, the piston will rise on its own over the next few minutes and you be required to repeat the entire reset process. .. figure:: /images/sample_removal/line_valve.png :alt: line_valve :width: 720px :align: center | .. warning:: The syringe pump is now located on the 16BMB hutch roof, which puts the hydraulic oil reservior and majority of the hydraulic line above the height of the PE press. The potential energy of the oil in the line is enough to push the bottom piston of the press upwards and, given enough time, to pop the piston completely out of the press body. The latter would cause a significant oil leak and result in a loss of at least 3 shifts of beamtime. After removing the sample and lowering the press piston, the line valve MUST BE CLOSED until the next sample is loaded and compressed. 19. Replace the bottom anvil using magnets. Ensure you do not accidentally load an US anvil in the bottom of the press—it should be a standard anvil.